Aca-Bio is a specialized chemical probe designed for the specific enrichment and detection of lysine acetoacetylation (Kacac). Its core architecture consists of a hydroxylamine group, a polyethylene glycol (PEG) linker, and a biotin tag. Functionally, the hydroxylamine group selectively reacts with the unique ketone carbonyl moiety of Kacac under physiological conditions to form a stable, covalent oxime bond. This specific targeting allows Aca-Bio to accurately label Kacac-modified peptides in complex biological samples. Subsequently, the attached biotin tag enables efficient isolation of these labeled peptides via streptavidin-mediated affinity purification. A critical feature of Aca-Bio is its pH-responsive reversibility; the oxime bond remains stable at neutral pH but undergoes controlled cleavage in acidic environments. This allows for the gentle and effective elution of enriched peptides, facilitating subsequent mass spectrometry analysis. By overcoming the limitations of traditional antibody-based methods, Aca-Bio serves as a highly specific and sensitive tool for global Kacac substrate profiling.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.