JT71 is a small-molecule inhibitor of the bacterial transcription regulator MrkH, showing potent anti-biofilm and anti-virulence activity in both in vitro and in vivo-relevant infection models. In vitro, JT71 inhibits MrkH-mediated transcription of the mrkA operon and suppresses type 3 fimbriae production in Klebsiella pneumoniae, resulting in ~50% reduction of mrkA promoter activity at 50 μM without affecting bacterial viability. It significantly reduces biofilm formation across multiple clinical and multidrug-resistant K. pneumoniae isolates as well as Citrobacter koseri, and downregulates both mrkH and mrkA expression, confirming dual transcriptional suppression. Mechanistically, JT71 binds to MrkH and interferes with c-di-GMP–dependent activation and/or DNA-binding activity, disrupting fimbriae assembly and surface adhesion.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.