Bohemine (50 mg/kg; intravenous injection; BALB/c mice) treatment shows Cmax is 72,308 nM, observed clearance is 0.23 L/h and T1/2 is 1.39 h[2]. Animal Model: BALB/c mice bearing the colon 26 murine tumor[2] Dosage: 50 mg/kg Administration: Intravenous injection (Pharmacokinetic Analysis) Result: Cmax is 72,308 nM, observed clearance is 0.23 L/h and T1/2 is 1.39 h.
In Vitro
Bohemine (0-30 µM; 72 hours; ME-750 cells) treatment inhibits cell growth. Addition of Bohemine at concentrations in the range of 1-10 µM results in a short-term arrest of growth and of monoclonal antibody production. The short-term suppression of cell functions is followed by a significant temporary increase of specific growth rate and of specific production rate[1]. Hybridoma cells are retarded both at the G1/S boundary and at the G2/M boundary, depending on Bohemine (0-30 µM) concentration[1]. T-lymphoblastic cell line CEM is treated by Bohemine, five proteins are found to be downregulated, namely α-enolase, triosephosphate isomerase, initiation factor 5A, and α- and β-subunits of Rho GDP-dissociation inhibitor 1. These proteins play significant roles in glycolysis, proteosynthesis, and in cytoskeleton rearrangement[1]. Bohemine inhibits growth of human tumor cell lines with an IC50 of 27 µM[2]. Cell Viability Assay[1] Cell Line: Mouse hybridoma ME-750 cells Concentration: 0 µM, 1 µM, 3 µM, 10 µM and 30 µM Incubation Time: 72 hours Result: At 10 µM and 30 µM concentrations, the viable cell count was significantly lower with respect to control, i.e., 77% and 48%, respectively.
Kinase Assay
Cell Assay
Animal Administration
References
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