BMP signaling agonist sb4 (0.05 μM-1 μM; 24 hours) induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions[1]. BMP signaling agonist sb4 (1 μM; 0-60 mins) enhances the efficacy of signaling at each concentration of rhBMP4 tested. This effect is most pronounced at low concentrations of rhBMP4, whereby sb4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4[1]. Western Blot Analysis[1] Cell Line: Primary mouse kidney epithelial cells (PRECs) Concentration: 0.05 μM-1 μM Incubation Time: 24 hours Result: Incresed p-SMAD-1/5/9 abundance in PRECs. Western Blot Analysis[1] Cell Line: BRE-Luc cells Concentration: 1 μM Incubation Time: 0min, 5 mins, 15 mins, 30 mins, 45 mins, 60 mins Result: Acted to stabilize p-SMAD-1/5/9 to enhance the transcriptional response.
Kinase Assay
Cell Assay
Animal Administration
References
[1]. Bradford STJ, et al. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds.J Biol Chem. 2019 Mar 1;294(9):3125-3136.
Return Policy
If you are in any way unsatisfied with your purchase, you may return any item(s) within 365 days of its original purchase date.
Please provide your Order Number in the email. We strive to reply to all email inquiries within one business day.
