To evaluate the efficacy of IWP-2 in vivo, 200 μL each of IWP-2-liposome or free liposome i separately injected into C57BL/6 mice intraperitoneally about 2 h before injection of a similar volume of either blue-dye-filled latex beads or E. coli DH5α. IWP-2 causes significant reduction in the uptake of blue beads as well as E. coli as assessed by CFUs in peritoneal lavage cells within 2 h. In addition, the levels of TNF-α and IL-6 in the lavage fluid of the corresponding mice are reduced by 2-4-fold compared with control values. Interestingly, IWP-2 even induces a considerable increase in secretion of the anti-inflammatory cytokine IL-10[4]. Pretreatment with IWP-2 significantly (P<0.05) abolished SP-induced increase of Wnt3a, p-GSK3β, and β-catenin expressions[5].
In Vitro
IWP-2, an inhibitor of WNT processing and secretion. IWP-2 significantly enhances the anti-proliferative effect of LEF. It is also obvious that the combination of LEF and IWP-2 could minimize the expression of β-catenin, c-Myc, Cyclin D1, Bcl2 and Bax to the largest extent compared with single agents[2]. Following treatment in the MKN28 cell line for four days, 10-50 μM IWP-2 significantly suppressed the proliferation of MKN28 cells (P<0.05). In addition, anchor-dependent and anchor-independent colony numbers are significantly decreased following IWP-2 treatment (P<0.05)[3].
Kinase Assay
Cell Assay
Animal Administration
References
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