| Description |
TOT-5, a tri-oleoyl-Tris ionizable lipid (pKa 6.2), enables splenic B cell-targeted mRNA delivery via 15% DSPC-incorporated LNPs. Its charge-neutral, hydrophobic surface minimizes hepatic ApoE uptake and enhances complement C3 adsorption, facilitating CD21/35-mediated uptake by marginal zone B cells. In vivo, intravenous 15%DSPC-LNPs showed 8-fold higher spleen-to-liver luciferase expression vs 3%DSPC, with anti-CD21/35 blocking 60% B cell uptake. Intramuscular administration induced robust OVA-specific IgG (10^5 titer) and CTL responses (3.5% tetramer+ CD8+ T cells) while reducing hepatotoxicity (ALT/AST levels ≤40 U/L vs SM-102-LNPs' 80-120 U/L). Cryo-ET confirmed stable lamellar structures (80-100 nm, ζ-potential -2 mV). This formulation achieves safe, ligand-free splenic targeting for mRNA vaccines. |
| Cat.No |
DC60821 |
| Name |
Lipid TOT-5 |
Chemical Properties
| CAS |
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| Formula |
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| MW |
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| Storage |
2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO |
References
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Splenic B cell-targeting lipid nanoparticles for safe and effective mRNA vaccine delivery-Yuichi Suzuki etl,Journal of Controlled Release |
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