2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO
Biological activity
Description
Target:
In Vivo
In Vitro
NSC 617145 (0.75-3 μM; 24-72 hours) shows maximal inhibition of proliferation (98%) at the lowest concentration in a WRN-specific manner in HeLa cells[1]. NSC 617145 (0.75 μM; 6 hours) induces WRN binding to chromatin and proteasomal degradation[1]. In FA-D2-/- cells, NSC 617145 (0.125 μM) acts synergistically with very low concentrations of Mitomycin C to inhibit proliferation in a WRN-dependent manner and induce double-strand breaks (DSB) and chromosomal abnormalities. NSC 617145 exposure results in enhanced accumulation of DNA-PKcs pS2056 foci and Rad51 foci in Mitomycin C-treated FA-deficient cells, suggesting that WRN helicase inhibition prevents processing of Rad51-mediated recombination products and activates NHEJ[1]. NSC 617145, induces cell cycle arrest and apoptosis in human T-cell leukemia virus type 1 (HTLV-1)-transformed adult T-cell leukemia cells[2]. Cell Viability Assay[1] Cell Line: HeLa cells Concentration: 0.75 μM, 1 μM, 1.5 μM, 2 μM, 3 μM Incubation Time: 24 hours, 48 hours, 72 hours Result: Inhibited cell proliferation in a WRN-specific manner. Western Blot Analysis[1] Cell Line: HeLa cells Concentration: 0.75 μM Incubation Time: 6 hours Result: Caused WRN to become degraded by a proteasome-mediated pathway.
Kinase Assay
Cell Assay
Animal Administration
References
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