DFHBI-1T Datasheet DC Chemicals
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Cat.No DC65702
Name DFHBI-1T

Chemical Properties

CAS 1539318-36-9
Formula C13H9F5N2O2
MW 320.214780569077
Storage 2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO

Biological activity

Description CAS NO.：1539318-36-9
Product Name：DFHBI-1T
Synonyms：DFHBI 1T;DFHBI-1T;(5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one;(5z)-5-(3,5-Difluoro-4-Hydroxybenzylidene)-2-Methyl-3-(2,2,2-Trifluoroethyl)-3,5-Dihydro-4h-Imidazol-4-One;(Z)-5-(3,5-Difluoro-4-hydroxybenzylidene)-2-methyl-3-(2,2,2-trifluoroethyl)-3,5-dihydro-4H-imidazol-4-one;Q27453430;O=C1N(CC(F)(F)F)C(C)=N\\C1=C/C1=CC(F)=C(O)C(F)=C1;4-[(Z)-3,5-Difluoro-4-hydroxybenzylidene]
EINEC:
Molecular Formula：C13H9F5N2O2
Molecular Weight：320.214780569077
Target:
In Vivo
In Vitro DFHBI-1T (20 μM; for 10 min) increases fluorescence in COS7 cells expressing (CGG)60-Spinach2 over DFHBI (20 μM)[1]. Broccoli-DFHBI-1T has ex/em=472 nm/507 nm and Spinach2-DFHBI-1T has ex/em=482 nm/505 nm[2].
Kinase Assay
Cell Assay
Animal Administration

References

[1]. Wenjiao Song, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201. [2]. Grigory S Filonov, et al. Broccoli: rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution. J Am Chem Soc. 2014 Nov 19;136(46):16299-308.
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