Digoxigenin (DIG) is a small molecule with high antigenicity. Similar to other commonly used haptens like 2,4 - Dinitrophenol (DNP) and biotin, it can be applied in a variety of biological detection methods. Generally, Digoxigenin is conjugated with large biomolecules to be detected, such as proteins and nucleic acids. Anti - Digoxigenin antibodies are directly or indirectly labeled with fluorescent dyes or enzymes for visualization and detection purposes. Under mild conditions, Digoxigenin maleimide can easily react with thiol groups, enabling the attachment of Digoxigenin molecules to proteins or thiol - substituted oligonucleotides.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.