HJ-4 is a novel piperine-derived small molecule that demonstrates potent and selective anti-tumor activity in colorectal cancer models. In vitro, HJ-4 significantly inhibits proliferation of HCT116 cells with an IC₅₀ of 15.82 µM—approximately 2.6-fold more potent than piperine—while maintaining low cytotoxicity toward normal 293T cells, indicating favorable tumor selectivity. At 8–32 µM, HJ-4 markedly suppresses colony formation, DNA synthesis, cell adhesion, migration, and invasion in HCT116 and SW480 cells, with superior efficacy compared to the parent compound. Additionally, it disrupts angiogenic network formation, reducing vascular structures to <50% of control levels at higher concentrations. In vivo, using a chicken embryo chorioallantoic membrane (CAM) model, HJ-4 produces dose-dependent tumor growth inhibition, achieving >70% reduction in tumor weight at 32 µM and significantly decreasing vascular density. These findings demonstrate that HJ-4 exerts robust anti-proliferative, anti-metastatic, and anti-angiogenic effects, supporting its potential as a promising lead compound for colorectal cancer therapy.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.