IRBM-Z-1 is a non-competitive allosteric inhibitor of the Zika virus (ZIKV) NS2B-NS3 protease, identified through phenotypic screening for ZIKV replication inhibitors. In vitro, IRBM-Z-1 inhibits recombinant ZIKV NS2B-NS3 protease with an IC50 of 1.8 ± 0.8 μM and binds the protease with a KD of 1.52 ± 0.75 μM. In ZIKV replicon assays, IRBM-Z-1 suppresses viral RNA replication with an EC50 of 6.0 ± 1.9 μM and inhibits ZIKV-induced cytopathic effects in cell culture with an EC50 of approximately 6.25 μM, while showing no significant cytotoxicity at concentrations up to 32 μM. Activity is strongly dependent on NS3 residue I156, with the I156T mutation markedly reducing potency.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.