OF-Deg-Lin is a biodegradable lipid containing an ester group, developed from the nonbiodegradable, linoleic acid derived OF-02; mRNA-LNPs containing OF-Deg-Lin showed high expression in the spleen. The ionizable lipid Of-Deg-Lin was synthesized, which possessed the similar chemical structure with OF-02.Both Of-Deg-Lin and OF-02 had diketopiperazine core and doubly unsaturated tails, and the difference between them was that Of-02 contained nondegradable 1,2-amino-alcohol linkages, whereas Of-Deg-Lin contained degradable ester linkages The change of linker altered iLNPs distribution and mRNA expression in vivo. Although the Of-Deg-Lin iLNPs could accumulate in the liver and spleen, they induced the expression of most functional proteins in B lymphocytes of spleen (over 85% of the total protein), not in the liver. Of-Deg-Lin iLNPs can deliver mRNA and express functional proteins in the spleen specifically, but the mechanism is unclear.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.