ATO 532 Acid has an identical molecular structure to ATTO 532 Acid. It is a fluorescent label related to the well - known dye Rhodamine 6G.
This dye is characterized by strong absorption, a high fluorescence quantum yield, excellent thermal and photostability, and good water solubility. Thanks to these features, ATO 532 is highly appropriate for single - molecule detection applications and high - resolution microscopy techniques, including Photoactivated Localization Microscopy (PALM), direct Stochastic Optical Reconstruction Microscopy (dSTORM), Structured Illumination Microscopy (SIM), and Stimulated Emission Depletion Microscopy (STED).
In addition, it is also very suitable for use in Fluorescence - Activated Cell Sorting (FACS) and Fluorescence In Situ Hybridization (FISH). The fluorescence of ATO 532 Acid can be most efficiently excited within the range of 515 - 545 nanometers.
