2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO
Biological activity
Description
CAS NO.：14003-96-4 Product Name：7-Hydroxy-4-methyl-2-oxo-2H-chromene-8-carbaldehyde Synonyms：7-Hydroxy-4-methyl-2-oxo-2H-chromene-8-carbaldehyde;4Μ8C;7-hydroxy-4-methyl-2-oxochromene-8-carbaldehyde;8-Formyl-4-methylumbelliferone;IRE1 Inhibitor III;4μ7-Hydroxy-4-methyl-2-oxo-2H-1-benzopyran-8-carboxaldehyde;4-Methyl-7-hydroxy-8-formylcoumarin;7-Hydroxy-8-formyl-4-methylcoumarin;8-Formyl-7-hydroxy-4-methyl-2H-1-benzopyran-2-one;4M8C;4u8C;4μIRE1 Inhibitor III, 4μ8C;8-Formyl-7-hydroxy-4-methylcoumarin;4;I8C;4mu8C;2H-1-Benzopyran-8-carboxaldehyde, 7-hydroxy-4-methyl-2-oxo-;4micro8C;HMS3743K09;HMS3653K06;BCP21011;STK504078;s7272;4150AD;BDBM5001379 EINEC: Molecular Formula：C11H8O4 Molecular Weight：204.1788
Target:
In Vivo
4μ8C reverses the ER stress-dependent loss of several known RIDD targets, with an EC50 of approximately 4 μM, approximating that of inhibition of XBP1 target gene activation[1].
In Vitro
When applies to the media of ER stressed cultured cells, 4μ8C inhibits Xbp1 splicing in a concentration-dependent manner. 4μ8C dissociates slowly from IRE1, but ishout of inhibitor leads to rapid recovery of Xbp1 splicing in cells[1].The IRE1 endoribonuclease inhibitor 4μ8c prevents the splicing of the XBP1 mRNA in response to ER stress caused by mutant proinsulin production[2]. The inositol-requiring enzyme 1α (IRE1α) is a serine-threonine kinase that plays crucial roles in activating the unfolded protein response. 4μ8C treatment dramatically inhibits IL-4 production by CD4+ T cells under Th0 conditions because both the IL-4 levels in the culture supernatant and the percentage of IL-4 positive cells are reduced by 4μ8C treatment. In addition, both IL-5 and IL-13 production are significantly reduced upon treatment with 4μ8C[3].
Kinase Assay
Cell Assay
Animal Administration
References
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