WST-8 for CCK-8 |CAS 193149-74-5|tetrazolium reagent

Cas No.:	193149-74-5
Chemical Name:	WST-8 sodium
Synonyms:	WST8,WST 8
SMILES:	O=S(C1=CC=C(C2=NN(C3=CC=C([N+]([O-])=O)C=C3)[N+](C4=CC=C([N+]([O-])=O)C=C4OC)=N2)C([S-](=O)([O])=O)=C1)(O[Na])=O
Formula:	C₂₀H₁₃N₆NaO₁₁S₂
M.Wt:	600.465
Purity:	>98%
Sotrage:	2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO

Description:	WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
In Vitro:	The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].
References:	[1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.

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