EUK-134

Cas No.:	81065-76-1
Chemical Name:	chloro[[2,2'-[1,2-ethanediylbis[(nitrilo-κN)methylidyne]]bis[6-methoxyphenolato-κO]]]-manganese
Synonyms:	EUK-134; EUK-134; EUK-134;
SMILES:	COC1=C2C(C=[N]3CC[N]4=CC5=CC=CC(OC)=C5O[Mn+3]34([O-2]2)[Cl-])=CC=C1
Formula:	C18H18ClMnN2O4
M.Wt:	416.74
Purity:	>98%
Sotrage:	Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term

Description:	EUK-134, a synthetic superoxide dismutase and catalase mimetic, protects rat kidneys from ischemia-reperfusion-induced damage. EUK-134 is a superoxide dismutase (SOD) mimetics (SODm) with catalase activity. EUK-134 is a mitoprotective antioxidant. EUK-134 reduces the expression of NF-κB, MDA level, and protein carbonylation in H9C2 cells[1][2][3].
Target:	NF-κB
In Vitro:	EUK-134 (10-300 μM) is able to improve cell viability and reduces Paraquat (1 mM)-induced cell death significantly via dismutation or scavenging of superoxide anions and reduces hydroxyl radical generation[2]. Higher concentrations of EUK-134 alone (30-300 μM) produces modest but significant reductions in cellular viability; however, this effect is not reflected in cell death measurements made at these concentrations[2]. EUK-134 is a salen-manganese complex with superoxide dismutase and catalase activity.Supplementation with EUK-134 is therefore identified as a novel approach to attenuate cardiac hypertrophy and lends scope for the development of EUK-134 as a therapeutic agent in the management of human cardiovascular disease.EUK-134 is a mitoprotective antioxidant with Mn-superoxide dismutase (Mn SOD) and catalase activity[3]. Cell Viability Assay[2] Cell Line: NRK-52E cells Concentration: 0, 1, 3, 10, 30, 100, and 300 μM Incubation Time: 24 hours Result: Co-incubation with Paraquat (1 mM) and increasing concentrations of EUK-134 (1-300 μM) resulted in a significant reduction in paraquat-induced cellular injury and death at 100 and 300 μM.
References:	[1]. P Gianello, et al. EUK-134, a synthetic superoxide dismutase and catalase mimetic, protects rat kidneys from ischemia-reperfusion-induced damage. Transplantation. 1996 Dec 15;62(11):1664-6. [2]. Mohamed Samai, et al. Comparison of the effects of the superoxide dismutase mimetics EUK-134 and tempol on paraquat-induced nephrotoxicity. Free Radic Biol Med. 2007 Aug 15;43(4):528-34. [3]. Sreeja Purushothaman, et al. Mitoprotective antioxidant EUK-134 stimulates fatty acid oxidation and prevents hypertrophy in H9C2 cells. Mol Cell Biochem. 2016 Sep;420(1-2):185-94.