Novel sialidase-activatable fluorescence probe with improved stability for the sensitive detection of sialidase

A potent, orally bioavailable, dual Src/Abl kinase inhibitor with IC50 of 0.5, 0.4, 0.5 and <1 nM for Src, Lck, Yes and Bcr-Abl, respectively.

PAMP-12(human, porcine) is a major component of immunoreactive (ir)-PAMP, is processed from the adrenomedullin precursor, is a potent hypotensive peptide and participates in cardiovascular control.

Cell permeable derivative of calcein, becoming fluorescent on hydrolysis

Fluo-3 is a cell-impermeable calcium ion (Ca2+) indicator. Fluo-3 itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm).

Fluo-3 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.

Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator.

MitoTracker Green FM is a green-fluorescent dye that can selectively accumulate in the mitochondrial matrix. MitoTracker Green FM covalently binds to mitochondrial proteins by reacting with free thiol groups of cysteine residues.

MitoMark Red I is a red fluorescent mitochondrial stain. Fluorescence intensity is dependent on mitochondrial membrane potential. Excitation/emission maxima λ ~ 578/599 nm.

ER-Tracker Red is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER).

ER-Tracker Green is a fluorescent dye that specific for endoplasmic reticulum.

LysoTracker Red (LysoTracker Red DND-99) is a paraformaldehyde fixable probe that concentrates into acidic compartments of cells and tissues. LysoTracker Red is an indicator of cell death in a manner similar to other standard assays (Annexin, terminal dUTP nick end labeling, Nile blue sulfate, neutral red, and acridine orange). LysoTracker Red is a marker for late endosomes and lysosomes.

Fluorescein (FAM) amine, isomerically pure dye (5-isomer). Unlike fluoresceinamine, this reagent contains a linker arm with an aliphatic amine group that is more reactive. It can react with electrophilic reagents like activated esters or epoxides, and be involved in enzymatic transamination.

5-FAM SE is a single isomer, it is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides.

The single isomer, 5-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition

OG 488 SE is a Green fluorescent dye; supplied as NHS ester. OG-488 SE is a potential fluorescein precursor for live bacteria detection.

EOS789 is a potent, pan-phosphate transporter inhibitor, inhibits phosphate uptake in a noncompetitive manner for human NaPi-IIb, PiT-1, and PiT-2 with IC50 of 6.8, 1.5 and 1.7 uM, respectively.

OG 488, acid, a fluorescent pH indicator, has many applications in biochemistry and neurosciences.

The amine-reactive 5-TAMRA, SE and its conjugates yield bright, pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ~546/579) with good photostability.

Tetramethylrhodamine (TMR, TRITC) has been a widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry.

GBT1118 is a potent and orally active allosteric modifier of hemoglobin oxygen affinity. GBT1118 increases tolerance to severe hypoxia.

Far red / near infrared dye alkyne for Click Chemistry labeling. Cyanine5.5 is an analog of Cy5.5, a popular fluorophore which has been widely used for various applications including intact organism imaging. This reagent can be conjugated with azido groups under mild copper catalyzed Click Chemistry conditions. This reagent is soluble in organic solvents, but mixtures of water with small percent of DMSO can be used for efficient conjugation. Cyanine5.5 alkyne can also be used for the labeling of small molecules with this far red/NIR dye.

Cyanine5.5 maleimide is a thiol reactive dye which is capable of selective labeling of sulfhydryl groups in proteins, an analog of Cy5.5? maleimide. Near infrared emission of Cyanine5.5 makes this dye suitable for bioimaging applications. Cyanine5.5 can replace Cy5.5?, Alexa Fluor 680, and DyLight 680.

CY5.5 carboxylic acid, also known as Cyanine5.5 carboxylic acid, is a Far-red emitting fluorescent dye with Ex/Em wavelength 673nm/707nm. CY5.5 carboxylic acid can be considered non-reactive for most applications and used as a control or reference sample, and for instrument calibration.

Cyanine5 alkyne for Click Chemistry, an analog of Cy5 alkyne. With this product, deeply colored, and photostable Cyanine5 fluorophore can be attached to various molecules via Click Chemistry reaction with azides. This alkyne is non-water soluble, but it can be dissolved in DMF or DMSO prior to reaction, and added to aqueous reaction mixture. With our labeling protocol, the labeling reaction is very efficient, and high-yielding. Various substrates bearing azides can be used for the labeling, including azido-labeled biomolecules, polymers, and solid surfaces.

Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates. Cyanine5 is an analog of Cy5?, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers. For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.

Non-activated carboxylic acid, an analog of Cy5? free carboxylic acid. Contains Cyanine5 fluorophore. This dye has limited water solubility, but can be dissolved in mixtures of water with organic phase (DMF, DMSO, alcohols) to obtain useful concentrations of the material in solution. Water-soluble version is available. This molecule can be considered non-reactive dye for the use in control samples, and for instrument calibration. For coupling with amines and protein labeling, consider using Cyanine5 NHS ester, or water-soluble sulfo-Cyanine5 NHS ester.

Sulfo-Cyanine 5 NHS Ester is a water soluble amine reactive red emitting fluorescent dye.Used for the labeling of various amine containing molecules in aqueous phase without use of any organic co-solvent. Useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.

Sulfo-Cyanine5.5 is a water soluble cyanine dye for far red / NIR applications such as in vivo imaging. The dye possesses four sulfonate groups that render it highly hydrophilic and water soluble. As well as other cyanines, sulfo-Cyanine5.5 has an outstanding extinction coefficient that makes it a bright fluorescent label for the far red region. This is an amine-containing fluorescent dye. The amine group is separated from the fluorophore by a relatively long linker that facilitates conjugation. The aliphatic primary amine group can be coupled with various electrophiles (activated esters, epoxides, etc), and also be used in enzymatic transamination labeling.

Water soluble reagent with alkyne group for Click Chemistry. Sulfo-Cyanine5 is a sulfonated dye, which possesses high hydrophilicity and aqueous solubility, exceptionally high extinction coefficient, good quantum yield, and compatibility with many instruments. This reagent is recommended for the conjugation with proteins, nanoparticles, and other applications where hydrophilicity is important.