Cell permeable derivative of calcein, becoming fluorescent on hydrolysis

Calcium Green 1AM is a cell-permeant fluorescent calcium indicator (Excitation 506 nm; Emission 531 nm). Calcium Green 1AM is converted to the fluorescent calcium indicator by intracellular esterases.

Rhod-2 potassium is a cell impermeant, red fluorescent calcium indicator. Rhod-2 potassium exhibits a significant shift in fluorescence intensity upon calcium binding (ex max=549 nm; calcium-free v. ex/em max=552/581 nm; calcium-bound). Unlike the UV-excitable indicators Fura-2 and Indo-1 (HY-D0121), there is no accompanying spectral shift.

Rhod-2 is a high-affinity visible light excitation wavelength Ca2+ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm.

Fluo-3 pentaammonium is a cell-impermeable calcium ion (Ca2+) indicator. Fluo-3 pentaammonium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm).

Fluo-3 pentapotassium is a cell-impermeable calcium ion (Ca2+) indicator. Fluo-3 pentapotassium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm).

Fluo-3 is a cell-impermeable calcium ion (Ca2+) indicator. Fluo-3 itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm).

Fluo-3 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.

Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator.

MitoTracker Green FM is a green-fluorescent dye that can selectively accumulate in the mitochondrial matrix. MitoTracker Green FM covalently binds to mitochondrial proteins by reacting with free thiol groups of cysteine residues.

MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm).

MitoMark Red I is a red fluorescent mitochondrial stain. Fluorescence intensity is dependent on mitochondrial membrane potential. Excitation/emission maxima λ ~ 578/599 nm.

ER-Tracker Red is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER).

ER-Tracker Green is a fluorescent dye that specific for endoplasmic reticulum.

C6 NBD Ceramide is a Golgi apparatus fluorescent probe with cell membrane permeability. C6 NBD Ceramide can be used for fast and convenient green fluorescent labeling of Golgi in living and fixed cells, and can be used to observe changes in Golgi morphology in living cells (Ex=466 nm, Em=536 nm). C6-NBD-ceramide is metabolized to fluorescent sphingomyelin and glucosylceramide, can be used for the study of sphingolipid transport and metabolic mechanism.

The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye. BODIPY TR Ceramide (Golgi-Red Tracke) is a Golgi-specific fluorescent dye, which can visualise individual cells[1]. Ex/Em=589 nm/616 nm.

The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye. BODIPY Fl C5-Ceramide is a Golgi-specific green fluorescent dye, which can visualise individual cells. Ex/Em= 505 nm/512 nm.

LysoTracker Red (LysoTracker Red DND-99) is a paraformaldehyde fixable probe that concentrates into acidic compartments of cells and tissues. LysoTracker Red is an indicator of cell death in a manner similar to other standard assays (Annexin, terminal dUTP nick end labeling, Nile blue sulfate, neutral red, and acridine orange). LysoTracker Red is a marker for late endosomes and lysosomes.

Green DND-26 is a green fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 504/511 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and generally gather on spherical organelles. Green DND-26 is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes.

Maleimide-5’FAM is a fluorescein. Maleimide-5’FAM can be used for labeling non-transduced cells.

Fluorescein (FAM) amine, isomerically pure dye (5-isomer). Unlike fluoresceinamine, this reagent contains a linker arm with an aliphatic amine group that is more reactive. It can react with electrophilic reagents like activated esters or epoxides, and be involved in enzymatic transamination.

5-FAM-Alkyne is a high selective and sensitive fluorescent biosensor for alkaline phosphatase (ALP). 5-FAM-Alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

5-FAM SE is a single isomer, it is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides.

The single isomer, 5-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition

OG 488 SE is a Green fluorescent dye; supplied as NHS ester. OG-488 SE is a potential fluorescein precursor for live bacteria detection.

OG 488, acid, a fluorescent pH indicator, has many applications in biochemistry and neurosciences.

2',7'-Difluorofluorescein (Oregon green 488) is a fluorescein derivative and a pH-sensitive fluorescent probe (pKa ~4.7). Upon excitation at 488 nm, 2',7'-Difluorofluorescein exhibits pH-sensitive fluorescence intensity through the formation of dianions, while its pH sensitivity decreases under excitation at 450 nm, allowing its use in ratiometric pH analysis. 2',7'-Difluorofluorescein can be used for the quantitative analysis of pH values in the range of 2-7 in submicron aerosol particles.