HMN-154

Cas No.:	173528-92-2
Chemical Name:	4-Methoxy-N-[2-(2-pyridin-4-ylethenyl)phenyl]benzenesulfonamide
Synonyms:	Benzenesulfonamide,4-methoxy-N-[2-[(1E)-2-(4-pyridinyl)ethenyl]phenyl]-;4-methoxy-N-[2-(2-pyridin-4-ylethenyl)phenyl]benzenesulfonamide;(E)-4-(2-(2-(N-(4-Methoxybenzenesulfonyl)amino)phenyl)ethenyl)pyridine;Benzenesulfonamide, 4-methoxy-N-(2-((1E)-2-(4-pyridinyl)ethenyl)phenyl)-;Benzenesulfonamide, 4-methoxy-N-(2-(2-(4-pyridinyl)ethenyl)phenyl)-, (E)-;(E)-4-[2-{2-[N-(4-Methoxybenzenesulfonyl)aMino]phenyl}ethenyl ]pyridine;HMN-154;4-methoxy-N-[2-[(E)-2-pyridin-4-ylethenyl]phenyl]benzenesulfonamide;AK625982
SMILES:	S(C1C([H])=C([H])C(=C([H])C=1[H])OC([H])([H])[H])(N([H])C1=C([H])C([H])=C([H])C([H])=C1C([H])=C([H])C1C([H])=C([H])N=C([H])C=1[H])(=O)=O
Formula:	C20H18N2O3S
M.Wt:	366.4335
Purity:	>98%
Sotrage:	2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO

Description:	HMN-154 is a novel benzenesulfonamide anticancer compound; inhibits KB and colon38 cells with IC50 values of 0.0026 and 0.003 μg/mL, respectively.
In Vitro:	HMN-154 interacts with NF-YB and thereby interrupts the binding of the NF-Y heterotrimer to DNA. NF-YB and thymosin β-10 are specific cellular binding proteins of HMN-154 and that this shared region is necessary for the binding to HMN-154. HMN-154 inhibits DNA binding of NF-Y to the human major histocompatibility complex class II human leukocyte antigen DRA Y-box sequence in a dose-dependent manner. HMN-154 shows very strong cytotoxicity against KB and colon38 cells with an IC50 value of 0.0026 and 0.003 μg/mL, respectively. HMN-154/BSA binds recombinant NF-YB or thymosin β-10 and the binding is inhibited by the addition of HMN-154 as the competitor. The binding between HMN-154 and NF-YB is specific and depends on its cytotoxicity[1].
Cell Assay:	Cells are seeded into a 96-well microplate at a cell density of 10000/well. Drug is added on the next day, and the plate then is incubated for 72 h at 37°C. The growth inhibitory concentration is measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay[1].
References:	[1]. Tanaka H, et al. Isolation of cDNAs encoding cellular drug-binding proteins using a novel expression cloning procedure: drug-western. Mol Pharmacol. 1999 Feb;55(2):356-63.