LM22B-10

Cas No.:	342777-54-2
Chemical Name:	2-[4-[[4-[Bis(2-hydroxyethyl)amino]phenyl]-(4-chlorophenyl)methyl]-N-(2-hydroxyethyl)anilino]ethanol
Synonyms:	LM22B-10;CBMicro_004706;QCXQLSGBOUUVNH-UHFFFAOYSA-N;SMSF0003215;BCP30139;LM22B 10;ZB0041;CB07009;AK00792418;4,4'-(4-Chlorobenzylidene)bis[N,N-bis(2-hydroxyethyl)aniline];2,2',2',2'''-[[(4-Chlorophenyl)methylene]bis(4,1-phenylenenitrilo)]tetrakisethanol;2-{[4-({4-[bis(2-hydroxyethyl)amino]phenyl}(4-chlorophenyl)methyl)phenyl](2-hydroxyeth;2-[4-[[4-[Bis(2-hydroxyethyl)amino]phenyl]-(4-chlorophenyl)methyl]-N-(2-hydroxyethyl)anilino]ethanol
SMILES:	ClC1C([H])=C([H])C(=C([H])C=1[H])C([H])(C1C([H])=C([H])C(=C([H])C=1[H])N(C([H])([H])C([H])([H])O[H])C([H])([H])C([H])([H])O[H])C1C([H])=C([H])C(=C([H])C=1[H])N(C([H])([H])C([H])([H])O[H])C([H])([H])C([H])([H])O[H]
Formula:	C27H33ClN2O4
M.Wt:	485.0149
Sotrage:	2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO

Description:	LM22B-10 is an activator of TrkB/TrkC neurotrophin receptor, and can induce TrkB, TrkC, AKT and ERK activation in vitro and in vivo.
In Vivo:	LM22B-10 (0.5 mg/kg) activates TrkB, TrkC, AKT and ERK in C57BL/6J mice. LM22B-10 (50 mg/kg, i,p,) shows increased phosphorylation at TrkBY817 and TrkCY820. LM22B-10 activates synaptic TrkB and TrkC and increases pre- and post-synaptic proteins and spine density in aged mice[1].
In Vitro:	LM22B-10 exhibits maximum neurotrophic survival activity levels that are higher than those maximally achieved with BDNF (53 ± 7.2% above BDNF at 0.7 nM) and NT-3 (91 ± 8.6% above NT-3 at 0.7 nM) with an EC50 value of 200-300 nM. LM22B-10 (1000 nM) induces neurites of significantly larger average lengths, up to -40 μM. LM22B-10 (250-2000 nM) binds to TrkB-Fc and TrkC-Fc in a dose-dependent manner. LM22B-10 inhibits binding of BDNF to TrkB-expressing cells and NT-3 to TrkC-expressing cells. LM22B-10 promotes cell survival and functions preferentially through TrkB and TrkC. LM22B-10, but not BDNF or NT-3, promotes neurite outgrowth in an inhibitory environment. LM22B-10 induces patterns of Trk and downstream signaling activation that are distinct from those of BDNF and NT-3. LM22B-10 also induces TrkB, TrkC, AKT and ERK activation in hippocampal neurons in culture[1].