NSC95397|NSC-95397|CAS 93718-83-3|DC Chemicals

Cas No.:	93718-83-3
Chemical Name:	2,3-bis[(2-Hydroxyethyl)thiol]-1,4-naphthoquinone
Synonyms:	NSC-95397;NSC 95397
SMILES:	C1=CC=C2C(=C1)C(=O)C(=C(C2=O)SCCO)SCCO
Formula:	C₁₄H₁₄O₄S₂
M.Wt:	310.38
Sotrage:	2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO

Description:

NSC 95397 is an inhibitor for dual-specificity phosphatases, including mitogen-activated protein kinase phosphatase-1 (MKP-1). NSC 95397 suppresses proliferation and induces apoptosis in colon cancer cells through MKP-1 and ERK1/2 pathway[1].

In Vitro:

NSC 95397 (0, 10, and 20 µM; 24 hour) decreases the cell viability of three colon cancer cell lines SW480, SW620, and DLD-1 in a concentration-dependent manner[1]. NSC 95397(10 μM; 24 hour) upregulates p21 while downregulates CDK4 and CDK6 were d in all three colon cancer cell lines SW480, SW620, and DLD-1 cells[1]. NSC 95397 (10 μM; 24 hour) reduces the phosphorylation of retinoblastoma protein (Rb) on Ser795 and Ser807/811[1]. NSC 95397 (20 μM; 24 hours) significantly increases cleaved caspase-9, -3, -7 and PARP levels[1]. NSC 95397 (10 μM; 6 hours) enhances the phosphorylation of its downstream ERK1/2 at Thr202/Tyr 204[1]. Cell Viability Assay[1] Cell Line: Human colon cancer cell lines, SW480, SW620, and DLD-1 Concentration: 0, 10, and 20 µM Incubation Time: 24 hours Result: The IC50 values of NSC 95397 for the cell growth of SW480, SW620, and DLD-1 cells were 9.9, 14.1 and 18.6 μM, respectively. Western Blot Analysis[1] Cell Line: SW480, SW620, and DLD-1 cells Concentration: 10 μM Incubation Time: 24 hours Result: p21 was upregulated while CDK4 and CDK6 were downregulated. Rduced the phosphorylation of Rb on Ser795 and Ser807/811

MSDS

COA

LOT NO.	DOWNLOAD

2018-0101
2018-0101
2018-0101

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