Hexadimethrine bromide

Cas No.:	28728-55-4
Chemical Name:	hexadimethrine bromide
Synonyms:	hexadimethrine bromide;sequa-brene;1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide,Polybrene;Hexadimethrine bromide,Polybrene;1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide;Polybrene
SMILES:	C[N+](CCCCCCC)(C)CCC[N+](C)(C)C.[Br-].[Br-]
Formula:	C39H90Br6N6
M.Wt:	1122.60000
Purity:	>98%
Sotrage:	2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO

Description:	Hexadimethrine bromide is a cationic polymer discovered to enhance retroviral transduction.
In Vitro:	Hexadimethrine bromide inhibits human mesenchymal stem cell proliferation during lentiviral transduction. Hexadimethrine bromide is considered non-toxic at low concentrations, but has been found to negatively affect cell proliferation in some cell types at concentrations greater than 10 µg/mL. Trypsinized cells exposed to Hexadimethrine bromide are visibly larger in size when viewed under the microscope[1].
Cell Assay:	Trypsinized cells are washed and resuspended in medium at 2×104 cells/mL with or without rhFGF-2 (final concentration=10 ng/mL, PeproTech) and with or without Hexadimethrine bromide at a final Hexadimethrine bromide concentration of 1, 4, or 8 µg/mL. The different conditions are seeded in 96-well plates at 1×103 cells in 50 µL per well in triplicate and cultured at 37°C, 5% CO2. After 6, 9, or 24 hr, the medium is changed and subsequent medium changes occurred every 3-4 days with 50 µL of ± FGF-2 medium (10 ng/mL). Plates are harvested on day 4, 7, 14, and 21 by removing the medium and placing the plates in the -80°C freezer until the day of analysis. The CyQUANT assay is then performed on the wells[1].
References:	[1]. Lin P, et al. Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction. PLoS One. 2011;6(8):e23891.