Norepinephrine hydrochloride|CAS 329-56-6|DC Chemicals

Cas No.:	329-56-6
Chemical Name:	(R)-4-(2-Amino-1-hydroxyethyl)benzene-1,2-diol hydrochloride
Synonyms:	(R)-4-(2-Amino-1-hydroxyethyl)benzene-1,2-diol hydrochloride;1,2-Benzenediol,4-[(1R)-2-amino-1-hydroxyethyl]-, hydrochloride (1:1);L-NORADRENALINE HYDROCHLORIDE;Noradrenaline hydrochloride;(-)-ARTERENOL HYDROCHLORIDE;(-)noradrenaline hydrochloride;(-)-norepinephrine hydrochloride;(R)-(-)-noradrenaline hydrochloride;[2-(3,4-dihydroxyphenyl)-2-hydroxy-ethyl]ammonium chloride;[2-(3,4-dihydroxyphenyl)-2-hydroxy-ethyl]azanium chloride;1-(3,4-dihydroxyphenyl)-2-aminoethanol hydrochloride;aktaminhydrochloride;L-ARTERENOL HYDROCHLORIDE;lev;L-NOREPINEPHRINE HYDROCHLORIDE;Norepinephrine hydrochloride
SMILES:	OC1=CC=C([C@@H](O)CN)C=C1O.Cl
Formula:	C₈H₁₁NO₃^.HCl
M.Wt:	205.63878
Purity:	>98%
Sotrage:	2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO

Description:	Norepinephrine (Levarterenol; L-Noradrenaline) hydrochloride is a potent agonist of adrenergic receptor (AR). Norepinephrine activates α1, α2, β1 receptors.
Target:	EC50: 5.37 μM (β1-selective adrenergic receptor)[1]
In Vitro:	Norepinephrine (NE) bitartrate monohydrate is generally considered to be a β1-subtype selective adrenergic agonist. Norepinephrine(NE) also has direct activity at the β2-adrenoceptor in higher concentrations[1]. Adipocytes from the inguinal fat pad (iWA) or the interscapular fat pad (BA) are isolated from neonatal wild-type C57BL/6J mice and cultured. To examine the effect of activating AT2 upon β-adrenergic signaling, cAMP production is first assessed in response to Norepinephrine (NE, 10 µM) with or without CGP (10 nM) co-treatment. Norepinephrine (NE) increases cAMP as expected in iWA, and CGP does not alter this effect. Norepinephrine (NE) is also known to induce lipolysis, and liberated fatty acids are required to functionally activate UCP1 protein and to stimulate heat production. CREB phosphorylation at Ser133 is increased after Norepinephrine (NE) treatment and significantly attenuated with CGP co-treatment in mouse iWA[2].
Cell Assay:	Subcutaneous preadipocytes derived from a 38-year old non-diabetic female donor are immortalized with TERT and HPV E6/E7. For the current studies, a stable diploid clone (referred to as clone B) with consistent differentiation capacity is isolated by ring cloning. Cells are grown in preadipocyte PGM2 media. Once cells are confluent, differentiation is induced by incubation in differentiation media consisting of dexamethasone, IBMX, indomethacin, and additional insulin. Cells are differentiated for 10 days. Prior to treatment, media is replaced with PGM2 media for one day and then switched to serum-free media overnight for treatments. Adipocytes are treated for 6 hours with vehicle, Norepinephrine (NE, 10 μM), CGP (10 nM), or Norepinephrine (NE) and CGP[2].
References:	[1]. MacGregor DA, et al. Relative efficacy and potency of beta-adrenoceptor agonists for generating cAMP in human lymphocytes. Chest. 1996 Jan;109(1):194-200. [2]. Littlejohn NK, et al. Suppression of Resting Metabolism by the Angiotensin AT2 Receptor. Cell Rep. 2016 Aug 9;16(6):1548-60.

MSDS

Cat. No.	Product name	Field of application
DC72880	Norepinephrine hydrochloride	Norepinephrine (Levarterenol; L-Noradrenaline) hydrochloride is a potent agonist of adrenergic receptor (AR). Norepinephrine activates α1, α2, β1 receptors.
DC72329	Upidosin	Upidosin (Rec 15/2739) is an α-1 adrenoceptor (α-1 AR) antagonist. Upidosin shows moderate selectivity for the α-1A AR subtype. Upidosin shows uroselectivity in urethra and prostate with a Kb value of 2-3 nM higher than in ear artery and aorta with a Kb value of 20-100 nM. Upidosin inhibits [3H]prazosin binding to cloned human α-1A adrenergic receptor. Upidosin can be used for the research of urethral obstruction.
DC49622	A-61603	A-61603 is a selective α1A-adrenergic receptor agonist. A-61603 increases the frequency of spontaneous Ca2+ transients in rat ventricular myocytes in vitro.
DC29046	Ritodrine hydrochloride	Ritodrine hydrochloride (DU21220 hydrochloride) is a β-2 adrenergic receptor agonist.
DC28370	Dabuzalgron	Dabuzalgron (Ro 115-1240) is an orally active and selective α-1A adrenergic receptor agonist for the treatment of urinary incontinence. Dabuzalgron protects against Doxorubicin-induced cardiotoxicity by preserving mitochondrial function.
DC28149	SR59230A hydrochloride	SR59230A hydrochloride is a potent, selective, and blood-brain barrier penetrating β3-adrenergic receptor antagonist with IC50s of 40, 408, and 648 nM for β3, β1, and β2 receptors, respectively.
DC28121	CGP 20712 A	CGP 20712 A (CGP 20712 mesylate) is a highly selective β1-adrenoceptor antagonist with an IC50 of 0.7 nM. CGP 20712 A exhibits ~10,000-fold selectivity over β2-adrenoceptors.
DC28102	SR59230A	SR59230A is a potent, selective, and blood-brain barrier penetrating β3-adrenergic receptor antagonist with IC50s of 40, 408, and 648 nM for β3, β1, and β2 receptors, respectively.
DC28084	Hydroxybupropion	Hydroxybupropion is the major active metabolite of Bupropion. Hydroxybupropion is metabolized by CYP2B6.Bupropion is an atypical antidepressant and smoking-cessation agent. Hydroxybupropion inhibits norepinephrine uptake with an IC50 value of 1.7 μM. Hydroxybupropion is also a nACh receptor antagonis tis more potent than Bupropion .
DC11346	Salbutamol (hydrochloride)	Salbutamol is an agonist of the β2-adrenergic receptor (β2-AR; Kd = 759 nM in a radioligand binding assay using CHO cells expressing the human receptor).